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轮虫线粒体基因组研究进展

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为了解轮虫线粒体基因组研究现状和存在的问题,通过对轮虫线粒体基因组研究相关文献的搜索、收集、阅读和归纳总结,通过对轮虫线粒体基因组序列进行分析和统计,提出轮虫线粒体基因组研究发展趋势及应用。随着分子生物学技术特别是测序技术的发展,轮虫线粒体基因组序列测定相关研究也逐步开展起来。目前已经报道的轮虫线粒体全序列有12个记录,共6种物种,主要集中于蛭态轮虫和臂尾轮属轮虫,蛭态轮虫线粒体基因组含1条环形染色体,臂尾轮属轮虫含两条环形染色体。所有轮虫线粒体基因组均含有基本的13个线粒体蛋白质编码基因,2个rRNA编码基因,22个tRNA编码基因(18~32个)。另外,少数轮虫如萼花臂尾轮虫含有额外的cytb基因拷贝。由于轮形动物中的轮虫体型微小,克隆培养困难,研究人员相对较少,轮虫线粒体基因组测序有待进一步深入。线粒体基因组研究中获得的序列可以用于轮虫系统发生关系研究、轮虫线粒体基因组比较学研究、轮虫隐种研究、轮虫分子鉴定研究、轮虫地理谱系学研究。
Research progress in the mitochondrial genome of species in rotifer
The research trend and application of rotifer mitochondrial genome research were proposed by search-ing,collecting,reading and summarizing relevant literature on rotifer mitochondrial genome research to under-stand the current situation and existing problems of rotifer mitochondrial genome research.Meanwhile,the research of analyzing and statistics of rotifer mitochondrial genome sequence were carried out.With the develop-ment of molecular biology technology,especially sequencing technology,research on mitochondrial genome sequencing in rotifers has gradually been carried out.Currently,there are 12 records of the complete mitochon-drial sequence of rotifers reported in 6 species,mainly concentrated in bdelloid rotifers and rotifers of the genus Brachionus.The mitochondrial genome of bdelloid rotifers contains one ring chromosome,while the rotifers of the genus Brachionus contain two ring chromosomes.The mitochondrial genome of all rotifers contains 13 basic mito-chondrial protein coding genes,2 rRNA coding genes,and 22 tRNA coding genes(18-32).In addition,a few roti-fers,such as B.calyciflorus,contain additional copies of the cytb gene.Due to the small size of rotifers and the dif-ficulty of cloning and culturing,relatively few researchers,and the mitochondrial genome sequencing of rotifers needs to be further deepened.The sequences obtained from mitochondrial genome research can be used for research on phylogenetic relationships of rotifers,comparative studies on mitochondrial genomes of rotifers,stud-ies on cryptic species of rotifers,molecular identification of rotifers,and geographic genealogy of rotifers.

rotifermitochondrial genomesequencing

何润泽、周利民、高炳淼、程双怀

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海南医学院,科学实验中心,海南海口 571199

海南医学院,药学院,海南海口 571199

轮虫 线粒体基因组 序列测定

海南省自然科学基金海南医学院人才引进科研启动基金

821RC568XRC190013

2024

水产学报
中国水产学会

水产学报

CSTPCD北大核心
影响因子:1.148
ISSN:1000-0615
年,卷(期):2024.48(8)