Exploration of candidate genes related to iridophore presenting blue skin in discus fish(Symphysodon haraldi)
The comparative transcriptomic analysis was performed on natural and albino blue discus with white discus fish(Symphysodon haraldi)respectively to excavate genes related to iridophores in blue skin.It was found a total of 2,192 different expression genes(DEGs)in natural blue fish,including 1,270 up-regulated and 922 down-regulated genes.The DEGs were primarily enriched in purine nucleotide biosynthetic process,actomyosin struc-ture organization,purine-containing compound biosynthetic process,oxidative phosphorylation,citrate cycle and other functions.For the albino blue discus fish compared to white one,there were 1,859 up-regulated and 1,309 down-regulated DEGs.Similarly,the dominant enriched pathways of these genes were oxidative phosphorylation,ribonucleoside diphosphate metabolic process,ADP metabolic process,cation binding,glycine,serine and threon-ine metabolism and other functions.In order to further explore candidate genes participating in iridophores appear-ing color,by comparing the database,a total of 32 and 38 DEGs were screened out in natural blue and albino blue discus,respectively.It could be predicted that alkal2b,gpnmb,fhl2 and pka were associated with the development of iridophores,andpgam,prtfdcl,pnp,slc23a1,slc2a9,rab38,rdh10,psat1,and paics might attend guanine syn-thesis and transportation in iridophores.This study might ground for in-depth analysis of the formation and regula-tion mechanism of blue structural color in fish.
万方数据
维普
