Objective To explore the protective effect and mechanism of tanshinone Ⅱ A on sex hormones and cell apopto-sis of testis following testicular torsion/detorsion in rates.Methods 64 male SD rats were randomly divided into sham operation group,model group,tanshinone Ⅱ A single and continuous treatment groups,with 16 rats in each group.Rats in the single treatment group were injected intraperitoneally with tanshinone Ⅱ A of 25 mg/kg after torsion/detorsion,while the continuous treatment group was maintained with tanshinone Ⅱ A of 25 mg/kg once per day for one week in addition to the above procedures.Rats from each group were killed at 4 and 29 days after reperfusion.The HE and TUNEL staining were used to observe testicular histopathological changes.Western blotting method was used to detect the expression of Bcl-2 and Bax.Sex hormones that Follicle stimulating Hor-mone(FSH),Luteinizing Hormone(LH)and Testosterone(T)were detected by ELISA.Results Compared with model group,less damage of testicular tissue and less germ cell apoptosis were observed in groups which received tanshinone Ⅱ A treatment(all P＜0.01),and the improvement was more pronounced in the continuous treatment group than in the single treatment group(P＜0.01).The continuous treatment group had higher expression of Bcl-2 and lower expression of Bax,compared with the single treat-ment group(all P＜0.01).There were no statistical significantly differences in the levels of sex hormones(FSH,LH,T)between the sham operation group and continuous treatment group(all P＞0.05),while there were statistical significantly differences among the sham operation group and model group,single treatment group(P＜0.01).Conclusion Tanshinone Ⅱ A can reduce the rat testicular ischemia-reperfusion injury after testicular torsion/detorsion by up regulate the expression of Bcl-2 and down regulate the expression of Bax,as well as maintain a dynamic balance among FSH,LH and T in serum.
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