Objective To investigate the expression and significance of microRNA-342-3p(miR-342-3p)and microR-NA-30a-5p(miR-30a-5p)in the serum of patients with acute ischemic cerebrovascular disease.Methods From March 2022 to March 2023,total 195 patients with acute ischemic cerebrovascular disease accepted by our hospital were regarded as acute ische-mic cerebrovascular disease group,and 103 healthy individuals were as reference group.Real time fluorescence quantitative PCR was used to detect the expression levels of serum miR-342-3p and miR-30a-5p.The Spearman method is used to analyze the diag-nostic value of correlation analysis,and the subject work characteristic curve is used to evaluate it.Results Compared with refer-ence group,acute ischemic cerebrovascular disease group showed a obvious increase in serum miR-342-3p and a obvious decrease in miR-30a-5p level(P＜0.05).There was a statistically obvious difference in low-density lipoprotein cholesterol(LDL-C),sys-tolic blood pressure,and diastolic blood pressure between acute ischemic cerebrovascular disease group and reference group(P＜0.05).There was no obvious difference in factors such as high-density lipoprotein cholesterol(HDL-C),triglycerides(TG),and total cholesterol(TC)(P＞0.05).There were obvious differences in serum miR-342-3p,miR-30a-5p,and NIHSS scores among patients with different neurological deficits(P＜0.05),moreover,serum miR-342-3p and NIHSS score in severe group were higher than those in the mild and moderate groups,while miR-30a-5p level was lower than that in the mild and moderate groups(P＜0.05).Serum miR-342-3p was positively correlated with NIHSS score(r=0.601,P＜0.05).Serum miR-30a-5p level was nega-tively correlated with the NIHSS score(r=-0.695,P＜0.05).The area under the curve(AUC)of serum miR-342-3p and miR-30a-5p and in combination for diagnosing severe neurological deficits in patients with acute ischemic cerebrovascular disease was 0.800,0.817,and 0.877,respectively,with sensitivity of 68.12％,73.91％,and 86.96％,respectively.The combined diag-nostic value of the two was higher than that of single diagnosis(Zcombination-miR-342-3p=2.081,P=0.038;Z combination-miR-30a-5p=2.026,P=0.043).Conclusion Serum miR-342-3p increases in patients with acute ischemic cerebrovascular disease,while the miR-30a-5p decreases.Both have certain diagnostic value for the degree of neurological dysfunction in patients with acute ischemic cerebrovascular disease.
维普
万方数据