NaCl胁迫下大毒滑锈伞菌对金银花生长及生理特性的响应
Growth and physiological mechanism study of H.crustuliniforme and Lonicera japonica symbiotic relationship under salt tolerance
慕德宇 1李楠 1王涛 2刘瑾 1慕宗昭 3张靖川4
作者信息
- 1. 山东建筑大学风景园林植物研究所,山东 济南 251010
- 2. 威海市城市管理综合服务中心,山东 威海 264200
- 3. 山东省林业外资与工程项目管理站,山东 济南 251014
- 4. 乳山市自然资源局林业产业发展中心,山东 威海 264200
- 折叠
摘要
以金银花(Lonicera japonica)和大毒滑锈伞菌(Hebeloma crustuliniforme)为材料,采用 0、60、90、120 mmol/L 共 4 个NaCl浓度的盐胁迫处理,研究了NaCl胁迫下植株根系的大毒滑锈伞菌定殖规律,探明了大毒滑锈伞菌对金银花盆栽苗生长及生理特性的作用机制.结果表明,随着盐浓度的升高,接菌组的脯氨酸含量比对照组的分别高 35.98%、141.28%、71.66%、8.79%,且在 90、120 mmol/L盐浓度下,大毒滑锈伞菌提高了金银花植株的耐盐性.经相关性和双因素方差分析发现,新梢生长量、分枝增长数与生物量呈显著正相关,脯氨酸与分枝增长数呈极显著负相关.盐胁迫下接种大毒滑锈伞菌能显著降低宿主苗高生长量和显著提高宿主脯氨酸含量,使金银花幼苗通过大量积累脯氨酸来抵御盐分胁迫.
Abstract
Lonicera japonica and Hebeloma crustuliniforme were used as materials in this study.0,60,90 and 120 mmol/L NaCl salt treatments were taken out to study the rule of H.crustuliniforme colonization in plant roots and analyze the mechanism of H.crustuliniforme on the growth and physiological characteristics of L.japonica seedlings.The results showed that with the increase in salt concentration,the proline content in the inoculated group plants was 35.98%,141.28%,71.66%and 8.79%higher than that of the control group,respectively.Under the treatment of 90 and 120 mml/L salt concentration,the salt tolerance of L.japonica plants was improved by the H.crustuliniforme.The results of correlation and two-way ANOVA indicated thatnew shoot growth and the number of branch growth were positively correlated with the biomass,and proline showed a highly significant negative correla-tion with the number of branch growth.H.crustuliniforme could significantly reduce the host seedling height growth and increase the host proline content significantly.The inoculation with H.crustuliniforme treatment could increase the proline accumulation of L.japonica seedlings to resist salt adversity under salt stress.
关键词
大毒滑锈伞菌/金银花/菌根真菌/生理特性/耐盐性Key words
Hebeloma crustuliniforme/Lonicera japonica/mycorrhizal fungi/physiological characteristics/salt resistance引用本文复制引用
基金项目
山东省农业科技资金(林业科技创新)项目(2019LY005)
出版年
2024
NETL
NSTL
万方数据