A novel electrochemical immunosensor capable of enzyme-free detection of alpha fetoprotein(AFP)is reported.This immunosensor uses the high catalytic properties of catalyzed gold-platinum nanoparticles against hydrogen peroxide and the high conductive performance of graphene to increase electron transfer capacity and signal amplification,escaping the need for enzymes in immunodetection,thus enabling enzyme-free detection.With a low detection limit at 0.000 03 pg/mL,this novel immunosensor worked well over the broad linear range of 0.000 1～10 pg/mL.
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