Kluyveromyces marxianus was used to ferment sugar beet to produce yeast cell and ethanol for bioenergy development.From a series of single factor tests and partial factorial experiment the fermentation conditions were preliminarily determined.The results showed that Kluyveromyces marxianus strain itself has cellulase and ligninase activity.When adding 0.1%cellulase,5 g/L ammonia urea,under the conditions of SSF and fed-batch culture,shaker speed 210.7 r·min-1,CaCO3 addition 5 g/L,and high cell density situation,the amount of yeast biomass and ethanol yield can reach a balanced higher value.
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