The traditional anticancer drugs are often chemical drugs,which are easy to cause drug resistance of cancer cells and have many side effects.In order to find new sources of anti-cancer drugs and explore whether the compatibility of Chinese herbal medicine can improve the original activity,we selected mastra appendicutata(D.Don)Makino,Actinidia arguta(Sieb & Zucc)planch.Ex miq,Anemone Raddeana Regel in the experiment.DPPH method was used to screen and analyze the antioxidant activity of the extracts from petroleum ether,chloroform,ethyl acetate,n-butanol and water.The results showed that the antioxidant activities of the extracts from the ethyl acetate layer and n-butanol layer were significantly higher than those of the extracts from other layers.The antioxidant activity of the extract of Chinese herbal medicine was higher than that of single extract.