β-环糊精金属有机骨架材料HPLC手性柱拆分噁唑禾草灵
Separation of Fenoxaprop Enantiomers by HPLC Column of β-cyclodextrin Metal-organic Framework Material
张家昌 1陆金富 1沙涛 1沈静茹1
作者信息
- 1. 中南民族大学 化学与材料科学学院 分析化学国家民委重点实验室,湖北 武汉 430074
- 折叠
摘要
合成了以β-环糊精和间羧基苯磺酰氯为有机配体、钾离子为配位离子的金属有机骨架材料(MOF)键合在高效液相色谱硅珠上,构建了高效液相色谱(HPLC)手性固定相(β-CD-MOF-P20@SiO2)新型材料,以此作为HPLC固定相,在反相色谱模式下,以乙酸铵-三乙胺/乙酸缓冲液和乙腈为流动相,优化流动相配比、缓冲液pH值、浓度和流速等色谱条件,获得了噁唑禾草灵两对映体最佳分离度,可达9.89.两对映体色谱峰响应信号与消旋体浓度在 6.20×10-6～2.99×10-4 mol/L范围内的线性相关性好(r=0.996 2～0.999 9),该MOF材料为噁唑禾草灵单一对映体定性、定量及制备提供了新方法.
Abstract
The synthesis of a novel material for chiral stationary phase in high-performance liquid chromatography(HPLC)by bonding β-cyclodextrin and meta-carboxybenzenesulfonyl chloride as organic ligands,with potassium ions as coordinating ions,onto silica beads(β-CD-MOF-P20@SiO2)was described.In reverse phase chromatography mode,using ammonium acetate triethylamine/acetic acid buffer and acetonitrile as the mobile phase,chromatographic conditions such as mobile phase ratio,buffer pH,concentration,and flow rate were optimized.Under the optimal separation conditions,the resolution reached 9.89.The linear correlation between the chromatographic peak response signals of the two enantiomer pairs and the concentration of the racemate was good in the range of 6.20×10-6 to 2.99×10-4 mol/L(r=0.996 2～0.999 9).A new method for the qualitative,quantitative determination and preparation of fenoxaprop-ethyl single enantiomer was developed
关键词
对映体分离/噁唑禾草灵/高效液相色谱柱材料/金属有机骨架材料/β-环糊精Key words
enantiomers separation/fenoxaprop-ethyl/high-performance liquid chromatography column material/metal-organic framework material/β-cyclodextrin引用本文复制引用
基金项目
中央高校基本科研业务费专项(CZY20036)
中央高校基本科研业务费专项(CZY19037)
出版年
2024
NETL
NSTL
万方数据