In this study,five ketones(2,3-butanedione,2,3-pentanedione,2,3-hexanedione,2,3-heptanedione and acetoin)in E-liquid were determined by Gas chromatography interfaced to mass spectrometry(GC—MS).E-liquid was dulited with methanol.After the oscillation extraction and filter,capillary column was used to seperate it.Five ketones in E-liquid were analysed by Gas chromatography interfaced to mass spectrometry(GC—MS)and quantified by external standard method.The results show that the five range of Coefficient of determination(R2)were more than 0.999,which mean the good linear relationships.Also,the retention time of these five ketones were less than 16 minutes.Compared with other methods,this pretreatment method is simple and efficient.The amount of five ketones can be accurately determined by different ratio of propylene glycol to glycerol.