Using Rudbeckia hirta L.water extract as reducing and stabilizer agent,silver nanoparticles with small particle size were synthesized under the Xenon lamp irradiation.The silver nanoparticles were characterized by infrared spectrum,UV-vis spectrum,X-ray diffraction and transmission electron microscopy.The bacteriostatic experiment of the silver nanoparticles was also studied and the results exhibit a good bacteriostatic effect on Escherichia coli and Staphylococcus aureus.