Determination of Yeast β-glucan in Milk Powder by High Pressure Acid Hydrolysis-high Performance Ion Chromatography
Objective:A method for the determination of yeast β-glucan in milk powder was developed by high pressure acid hydrolysis and ion exchange chromatography.Methods:The yeast β-glucan in milk powder was first enriched,and then was hydrolyzed into glucose by high pressure acid,which was analyzed by ion chromatography.The content of yeast β-glucan was calculated by formula conversion.Results:The linear relationship between the peak areas and concentration of glucose in 2～200 mg/L was good,which the correlation coefficient was 0.9998.The quantitative limit of yeast β-glucan was 11.1 mg/100 g.The recoveries of yeast β-glucan at three spiked levels were in the range of 81.5%～99.8%,and the relative standard deviations were all bellow 5%.Conclusion:The study indicated that the method was simplicity,efficiency,high sensitivity and accuracy,which was suitable for the determination of yeast β-glucan in milk powder.
high pressure acid hydrolysision exchange chromatographymilk powderyeast β-glucan
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