NIR Spectroscopy Combined with siPLS Method for Rapid Detection in Enzymatically Hydrolyzed Protein Milk Powder's Adulteration
Objective:This study aims to develop a rapid detection method for the adulteration of common protein powder in enzymatically hydrolyzed protein milk powder.Methods:The whey protein powder and plant protein powder were mixed in specific proportions into enzymatically hydrolyzed protein milk powder,resulting in 171 adulterated samples.NIR spectra were then collected.The obtained spectral data were divided into calibration and prediction sets using the SPXY method in a 3∶1 ratio.The adulteration detection model was established using the siPLS method and preprocessed with various methods.Results:The siPLS model established through SG first-order derivative preprocessing exhibited the best performance.The combined interval spectral range was[1135 to 1239.5,1660 to 1764.5,2080 to 2184.5 nm].The calibration model had a correlation coefficient(R2)of 0.9948,with an RMSECV value of 0.0101,while the prediction model had an R2 of 0.9945,with an RMSEP value of 0.0110,and an RPD of 13.5.Conclusion:Modeling with siPLS method can improve the stability and prediction accuracy.The method is simple to operate and can be applied for the rapid non-destructive detection of adulterated protein powder in enzymatically hydrolyzed protein milk powder.
near-infrared spectroscopyenzymatically hydrolyzed protein milk powder adulterationsynergy interval Partial Least Square(siPLS)milk powder adulteration
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