Study on the Ldentification Method of Thin Layer Chromatography(TLC)in the Material Reference of Classical Prescription Yunv Jian Decoction
Objective:To establish TLC identification methods of Rehmanniae Radix Praeparata,Anemarrhenae Rhizoma,Achyranthis Bidentatae Radix in the material reference of classical Yunv Jian decoction.Methods:TLC method the identification of rehmannia glutinosa and anemarrhena asphodeloides in Yunv Jian decoction was established by using verbascoside,rehmannia glutinosa and mangiferin,anemarrhena asphodeloides as the control.TLC method the identification of achyranthes bidentata in Yunv Jian decoction was established by using oleanolic acid and achyranthes bidentata as the control.The methods were verified.Results:The TLC identification method of Rehmanniae Radix Praeparata,Anemarrhenae Rhizoma,Achyranthis Bidentatae Radix,has the characteristics of clear spots,good resolution and no interference.Conclusion:This method is easy to operate and the results are accurate,and can be used as an identification method for the identification of three substances,and is used for the quality control of the material benchmark of Yunu decoction.
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