Anti-inflammatory Effect and Mechanism of Pomegranate Flower Polysaccharide on LPS-induced RAW 264.7 Cells
Objective:To investigate the anti-inflammatory effect and mechanism of pomegranate flower polysaccharide in RAW 264.7 macrophage inflammation model of mice induced by Lipopolysaccharide(LPS).Methods:The effects of different concentrations of pomegranate flower polysaccharide on the activity of RAW 264.7 cells were detected by CCK8 assay.The cell inflammation model was established after LPS stimulation,and 250,125,31.25 μg/mL pomegranate polysaccharide was added for treatment.After 24 h,NO levels in cell supernatant were detected by Griess method,and TNF-α,IL-1β,IL-6 and PGE2 levels in cell supernatant were detected by ELISA method.The mRNA expressions of iNOS,COX2,MyD88 and NF-κB were detected by RT-qPCR.Results:When the mass concentration of pomegranate flower polysaccharide was 0.25 mg/mL,the cell survival rate decreased,and when the mass concentration was below 0.125 mg/mL,the cell viability of RAW 264.7 increased.Compared with model group,the levels of NO,TNF-α,IL-1β,IL-6 and PGE2 in cell supernatant of pomegranate flower polysaccharide group were decreased,and the mRNA expressions of iNOS,COX2,MyD88 and NF-κB in RAW 264.7 cells were decreased(P＜0.05).Conclusion:Pomegranate flower polysaccharide can inhibit MyD88/NF-κB signaling pathway,reduce the level of NO release and inflammatory factors,and then reduce the inflammatory response of mouse macrophages induced by LPS.
万方数据
维普
