Inhibition of Xanthine Oxidase Activity and Its Combination with Diosmetin
The main difference of the structure of diosmetin,luteolin,albumin and apigenin is the difference of the substituents in the B ring.The inhibitory effect of R,on the enzyme was stronger than that of R2 through the activity of inhibiting xanthine oxidase(XO).In addition,when R,and R2 groups were present at the same time,R1 was a hydrophilic group and R2 was a hydrophobic group,which was more conducive to the inhibitor to reduce the enzyme activity.Diosmetin had the best inhibitory effect on enzymes.The inhibition mechanism of diosmetin and xanthine oxidase could be determined by UV and fluorescence spectroscopy.The reaction experiment with iron ion showed that geranyl could react with Fe2+of Fe-S catalytic active center of XO.The results of three-dimensional and synchronous fluorescence experiments showed that diosmetin mainly changed the microenvironment around tryptophan residues of the enzyme to experiment the effect of inhibiting the enzyme.Combined inhibition experiments showed that the inhibitor and allopurinol could synergically inhibit xanthine oxidase.It was of great significance to find new safe and non-toxic xanthine oxidase inhibitors.
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