Determination of Paeoniflorin in Shaogui Huoxue Yigan Pills by HPLC
Objective:Determination of paeoniflorin content in Shao Gui activating blood and benefitting liver pills by HPLC method.Methods:Utilizing High-Performance Liquid Chromatography(HPLC),the mobile is composed of acetonitrile and 0.05％phosphoric acid(17∶83,volume ratio).An Agilent TC-C18 column(4.6 nm×250 nm,5.0 μm)is used as the chromatography column.The column temperature is maintained at 40 ℃.The flow rate is set to 1.0 mL/min,and the detection wavelength is 230 nm.The injection volume for the samples is 10 μL.Resμlts:A good linear relationship is observed between the sample volume of paeoniflorin and the peak area within the range of 0.626 5 to 3.132 4 μg/mL.Linear regression equation y=437 393x-26 315,correlation coefficient r=1(n=6).This method has good specificity,precision,stability,and high repeatability.The recovery rate of sample addition is 96.23％,and the precision is 0.97％.Conclμsion:By measuring the content of three batches of samples,the tentative dosage of Shaogui Huoxue Yigan Wan containing white peony and paeoniflorin(C23H28O11)should not be less than 30.0 mg.Verification shows that this method is simple,fast,highly sensitive,and has good accuracy and reproducibility,Being able to accurately analyze the content of paeoniflorin in Shaogui Huoxue Yigan Wan,and can be used as a qualitative and quantitative method for paeoniflorin in Shaogui Huoxue Yigan Wan.
万方数据
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