Toxic Effects of Lead Pollution in e-waste Dismantling Area on Eisenia foetida
This study investigated the effects of Pb-contaminated soil at different concentration(0,100,250,500,1 000 and 2 500 mg/kg)on survival rate,growth inhibition rate,and the activity of antioxidant system enzymes(catalase,peroxidase,and glutathione transferase)and digestive enzymes(acid phosphatase and alkaline phosphatase)of earthworms(Eisenia foetida).The results showed that there was no significant effect on the survival and growth of earthworms in the 7-day acute exposure test.During the 14-day acute exposure test,Pb significantly inhibited the growth of Eisenia foetida when w(Pb(NO3)2)was 2 500 mg/kg.The interaction between exposure concentration and exposure time had a significant effect on the growth inhibition rate of Eisenia foetida.In the 14-day exposure test,when w(Pb(NO3)2)was 1 000 and 2 500 mg/kg,Pb significantly inhibited two digestive enzymes(ACP enzyme,AKP enzyme)and three antioxidant enzymes(POD enzyme,CAT enzyme,GST enzyme)in Eisenia foetida.This study comprehensively evaluated the toxic effects of lead on earthworms from individual level to molecular level,providing a new theoretical basis for monitoring soil lead pollution in e-waste dismantling area.