UPLC-MS/MS法同时测定化妆品中47种抗感染类药物
Simultaneous Determination of 47 Anti-infective Drugs in Cosmetics by UPLC-MS/MS
霍甜甜 1李宜鲜 1蒋培 1刘怡畅 1杨秋红1
作者信息
- 1. 河南省药品医疗器械检验院(河南省疫苗批签中心),河南郑州 450009
- 折叠
摘要
建立超高效液相色谱串联质谱法(UPLC-MS/MS)同时测定三种基质(水剂、乳剂、膏霜)化妆品中47种抗感染类药物的分析方法.样品加饱和氯化钠使分散,经甲醇超声提取后上机检测,各组分以基质标准曲线外标法定量.结果47种抗感染类药物在各自线性浓度范围内线性关系良好,检出限为0.02～0.47 mg/kg,定量限为0.10～2.37 mg/kg,3个加标水平平均回收率为80.7％～119.8％,相对标准偏差(RSD)均小于10％.本法具有预处理简便,响应度高,准确可靠等优点,可用于化妆品中47种抗感染类药物快速准确地定性筛查与定量测定.
Abstract
To simultaneous determine 47 kinds of anti-infective drugs in cosmetics with three matrix types(water,emulsion,cream)by ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The sample was dispersed by saturated sodium chloride,and then was ultrasonically extracted with methanol.And each component was quantified by an external standard curve with matrix.The results:47 kinds of anti-infective drugs showed good linear relationships in their respective linear concentration ranges.The limits of detection(LODs)were 0.02～0.47 μg/kg,and the limits of quantitation(LOQs)were 0.10～2.37 μg/kg.The average recoveries at three spiked levels were 80.7％～119.8％,with the relative standard deviations(RSD)not greater than 10.0％.This method has the advantages of simple pretreatment,high response,accuracy and reliability.It can be used for the rapid and accurate qualitative screening and quantitative determination of 47 anti-infective drugs in cosmetics.
关键词
UPLC-MS/MS/化妆品/抗感染类药物/同时测定Key words
UPLC-MS/MS/cosmetics/anti-infective drugs/simultaneous determination引用本文复制引用
基金项目
河南省市场监督管理局科技计划项目(2022sj83)
出版年
2024
NETL
NSTL
万方数据