Simultaneously Detecting of Vitamin B1 and Vitamin B6 in Compound four-Dimensional Ligustrine Capsule
Objective:To establish the method of simultaneously detecting of vitamin B1 and vitamin B6 in compound four-dimensional Ligustrine capsule by HPLC.Methods:Chromatographic analysis was performed on chromatographic column of C18(4.6 mm×250 mm,5 µm)with mobile phase of acetonitrile-sodium heptane sulfonate solution(0.32 g sodium heptane sulfonate,add 800 mL water to dissolve,add 2 mL triethylamine,10 mL acetic acid,dilute with water to 1 000 mL)(7:93).The flow rate was 1.0 mL/min with the detection wavelength at 274 nm and the colum temperature at 40 ℃.The injection volume is 10 µL.Results:Vitamin B1 has a good linear relationship in the range of 0.498 8～99.76 µg,and the regression equation Y=12 976x-2190.4(R2=1);Vitamin B6 has a good linear relationship in the range of 0.482 2～96.44 µg,and the regression equation Y=11071x+1 567(R2=1).The recovery rate of Vitamin B1 was 100.76％,and relative standard deviation(RSD)was 0.7％(n=9),Vitamin B6 was 99.51％,and relative standard deviation(RSD)was 0.8％(n=9).Conclusion:The method of HPLC is simple,rapid and accurate,and it's a scientific method for the determination of vitamin B1 and vitamin B6 in compound four-dimension Ligustrum capsule.
chromatography of high pressure liquidcompound four-dimension Ligustrum capsulevitamin B1vitamin B6content determination
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