Determination of Three Ginsenosides in Ginseng Oral Liquid by High Performance Liquid Chromatography-Diamide-Based Column with HILIC Mode
The hilic model of high performance liquid chromatography-amide column was established to determine the content of ginseng oral liquid.The chromatographic column was Venusil HILIC(4.5 mm×250 mm).Equalt elution was performed with water and acetonitrile as mobile phase.By establishing a mathematical model,the whole process of measurement was analyzed.The result showed that,Ginsenoside Re,ginsenoside Rg1,and ginsenoside Rb1 had good linear relationships in the range of 10.0～500 µg/mL,and the correlation coefficients(r2)were all greater than 0.999 8,RSD of precision experiments were 1.01％,1.21％and 1.05％,respectively.The recovery rate of the three ginsenosides were good.This study provided reference for the detection of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in ginseng oral liquid.
high performance liquid chromatography-diamamide column HILIC modeginseng oral liquidginsenosides
NETL
NSTL
万方数据
