高效液相色谱-二酰胺基色谱柱HILIC模式下对人参口服液中三种人参皂苷的测定
Determination of Three Ginsenosides in Ginseng Oral Liquid by High Performance Liquid Chromatography-Diamide-Based Column with HILIC Mode
黄晓艳 1于晓慧 2张萍1
作者信息
- 1. 方圆标志检验检测(山东)有限公司,山东济南 250100
- 2. 山东特检科技有限公司,山东济南 250000
- 折叠
摘要
建立高效液相色谱-酰胺基色谱柱HILIC模式,对人参口服液进行含量测定.采用Venusil HILIC为色谱柱(4.5 mm×250 mm),以水和乙腈为流动相进行等度洗脱,建立数学模型,分析整个测定流程.结果表明,人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1在10.0～500μg/mL范围内线性关系良好,相关系数(r2)均大于0.999 8,系统精密度实验R.SD分别为1.01％,1.21％,1.05％,三种人参皂苷的回收率均较好.研究为人参口服液中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的检测提供参考和借鉴.
Abstract
The hilic model of high performance liquid chromatography-amide column was established to determine the content of ginseng oral liquid.The chromatographic column was Venusil HILIC(4.5 mm×250 mm).Equalt elution was performed with water and acetonitrile as mobile phase.By establishing a mathematical model,the whole process of measurement was analyzed.The result showed that,Ginsenoside Re,ginsenoside Rg1,and ginsenoside Rb1 had good linear relationships in the range of 10.0～500 µg/mL,and the correlation coefficients(r2)were all greater than 0.999 8,RSD of precision experiments were 1.01％,1.21％and 1.05％,respectively.The recovery rate of the three ginsenosides were good.This study provided reference for the detection of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in ginseng oral liquid.
关键词
高效液相色谱-二酰胺基色谱柱HILIC模式/人参口服液/人参皂苷Key words
high performance liquid chromatography-diamamide column HILIC mode/ginseng oral liquid/ginsenosides引用本文复制引用
出版年
2024
NETL
NSTL
万方数据