Determination of Exogenous DNA Residual in Amino Acids by Fluorescence Staining
Objective:To establish a method for determination of DNA residual in 5 Amino acids by fluorescence staining.Methods:Determined by fluorescent enzyme labeling,fluorescent dye was specifically combined with double-stranded DNA,the excitation wavelength was 488 nm and the detection wavelength was 520 nm.Results:The assay was linear over the concentration range of 0.5～40 ng/mL with a correlation coefficient(r)of 0.999 9,the limit of quantitation was 0.55 ng/mL,the recovery of different amino acids varieties ranged from 73％～132％(n=9).Conclusion:This method is simple,rapid and suitable for the determination of DNA residual in different amino acids,which provides the technical basis for quality control.
amino acidsfluorescence staining methodexogenous DNA
万方数据
维普
