Determination of Vitamin B1 Content in Nourishing Pills
A method for determining vitamin B,in nourishing pills was established by high-performance liquid chromatography.C18 column(250 mm×4.6 mm,5 μm)was used with 0.05 mol/L sodium acetate solution-methanol(Vsodium acetate solution∶Vmethanol=65:35)as the mobile phase at the flow rate of 1.0 mL/min,column temperature was room temperature.The results showed that vitamin B,had a good linear relationship in the concentration range of 0～1.0 μg/mL(r=0.999 7).In precision validation,the RSD of peak area was 1.31％,and the RSD of retention time was 0.178％,the RSD in the accuracy experiment verification was 1.17％,the RSD in the repeated experimental verification was 0.779％.In the development process of different drying times,the deviation of stability content determination was 1.05％.The study provided reference and inspiration for the determination of vitamin B1 content and the development process of specialty foods.
high performance liquid chromatographyvitamin B1content determinationnourishing pills
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