The extraction and determination of polyphenols from Cucumis Metuliferus were studied in the paper.Ultrasonic-assisted extraction method was used to extract polyphenols from Cucumis Metuliferus.Orthogonal experimental method was used to confirm the optimal extraction process of polyphenols in Cucumis Metuliferus.The content of the total polyphenols in Cucumis Metuliferus was determined by Ultraviolet-Visible Spectrophotometry with gallic acid as reference substance in an alkaline environment.The absorbance and mass concentration were a good linear relationship at the mass concentration of gallic acid within the range of 0.02～0.10 mg/mL.The regression equation was Y=10.19X+0.001 100,R2=0.999 4.The optimal extraction conditions for polyphenols from Cucumis Metuliferus were extraction time 50 min,material-liquid ratio 1∶50(g∶mL)and ethanol concentration 60％.
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