Objective:To optimize the extraction process of total flavonoids from Rubus idaeus L.leaves by Box-Behnken response surface methodology.Methods:On the basis of single factor experiment,four factors including ethanol concentration,ultrasonic time,ultrasonic power and liquid-material ratio were investigated,Box-Behnken response surface methodology was used to optimize the extraction process of total flavonoids from Rubus idaeus L.leaves.Results:The optimum extraction conditions were ethanol concentration 75％,extraction time 50 min,ultrasonic power 260 W,ratio of liquid to material 50∶1(mL∶g).Under these conditions,the content of total flavonoids in Rubus idaeus L.leaves was 40.05 mg/g.Conclusion:The method is simple,stable and feasible,and can be used for the extraction of total flavonoids from Rubus idaeus L.leaves.
关键词
Box-Behnken响应面/覆盆子叶/总黄酮/提取工艺
Key words
Box-Behnken response surface/Rubus idaeus L.leaves/total flavonoids/extraction process
维普
万方数据