Studies on Optimization of Extraction Process for Total Alkaloids from Salsola collina Pall
The extraction rate total alkaloids from Salsola collina was used as the indicator,the extraction process of total alkaloids from S.collin was optimized through single factor test and response surface methodology,and DPPH and hydroxyl radical clearance was confirmed the antioxidant capacity of S.collin total alkaloids.The results showed that the optimal extraction process conditions for S.collin total alkaloids were respectively the liquid-material ratio of 36∶1(mL∶g),ultrasonic extraction time 60 min,compound enzyme addition 2％,enzymolysis time 1.4 h,ethanol volume fraction 57％.Under this condition,the extraction rate of the total alkaloids can reach 0.698 2％.The antioxidant activity experiment showed that the antioxidant activity increased with the concentration of alkaloids,when the S.collin total alkaloids concentration of 0.5 mg/mL,the DPPH radical and hydroxyl radical clearance reached 84.34％and 75.69％,respectively.This study optimized the extraction process of the total alkaloids in Salsola collina,preliminarily analyzed the antioxidant activity of the alkaloids,and provided a scientific basis for the analysis of the effective medicinal components of Salsola collina and the development of functional food.
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