Endogenous APE1 Assisted DNA Nanoprobe for in Situ Imaging Detection of miR-21
It is beneficial for improving the accuracy of tumor disease diagnosis and detection by using miRNA as a target for in situ imaging of tumor cells.However,limited by the low abundance of intracellular miRNA and the complex physiological environment within cells,the detection sensitivity of traditional nucleic acid probes'one-to-one signal output mode is low and prone to false positive signals,significantly compromising the performance in in situ detection.Thus,a walker-based DNA nanoprobe activated by miR-21 and APE1 was developed using gold nanoparticles as a carrier.By using this DNA nanoprobe,high sensitivity and specificity in situ imaging of tumor cells was successfully achieved,and false positive signals were effectively reduced.
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维普
