气相色谱法测定维生素预混料中肌醇的含量

Determination of Inositol Content in Vitamin Premix by Gas Chromatography

蓝燕玲 ¹潘云山 ¹温才洁 ¹张丽 ¹李雪莹 ¹黄嘉颖 ¹江子荣 ¹方丽¹

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作者信息

1. 广东省科学院测试分析研究所(中国广州分析测试中心),广东省化学测量与应急检测技术重点实验室,广东省保健食品功效成分检测与风险物质快速筛查工程技术研究中心,广东广州 510070
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摘要

建立了气相色谱法测定维生素预混料中肌醇含量的方法.维生素预混料样品中的肌醇经过水溶液提取,氮气吹干,采用硅烷化试剂进行衍生反应后,再采用正己烷提取,最后在气相色谱仪上进行分析,外标法定量.结果表明,肌醇在2～200 µg/mL质量浓度范围内线性关系良好,相关系数(R2)大于0.999;检出限(LOD)为0.04 mg/g,定量限(LOQ)为0.12 mg/g;相对标准偏差(RSD)为0.43％;在3个浓度添加水平(1.0,3.0,10.0 mg/g)内,回收率为95.0％～98.1％.该方法快速、准确、稳定,能够满足维生素预混料中肌醇含量的测定.

Abstract

A quantitative analysis and detection method for inositol in vitamin premixes using gas chromatography has been established.The inositol in the vitamin premix sample is extracted by aqueous solution,then dried and concentrated by nitrogen gas blowing,silylated,extracted by n-hexane,and finally analyzed by gas chromatography with external standard method for quantification.The results indicate that the linear relationship of inositol is good within the concentration range of 2～200 μg/mL,and the correlation coefficient(R2)is greater than 0.999.The detection limit(LOD)is 0.04 mg/g and the quantification limit(LOQ)is 0.12 mg/g.The relative standard deviation(RSD)is 0.43％.Within three concentration levels(1.0,3.0,10.0 mg/g),the recovery rate ranges is from 95.0％to 98.1％.This method is fast,accurate,and stable,and can meet the determination of inositol content in vitamin premixes.

关键词

气相色谱法/维生素预混料/肌醇/硅烷化衍生

Key words

gas chromatography/vitamin premix/inositol/silylation derivatization

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出版年

2024

山东化工

山东省化工研究院山东省化工信息中心

山东化工

影响因子：0.249

ISSN：1008-021X

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