Determination of Inositol Content in Vitamin Premix by Gas Chromatography
A quantitative analysis and detection method for inositol in vitamin premixes using gas chromatography has been established.The inositol in the vitamin premix sample is extracted by aqueous solution,then dried and concentrated by nitrogen gas blowing,silylated,extracted by n-hexane,and finally analyzed by gas chromatography with external standard method for quantification.The results indicate that the linear relationship of inositol is good within the concentration range of 2～200 μg/mL,and the correlation coefficient(R2)is greater than 0.999.The detection limit(LOD)is 0.04 mg/g and the quantification limit(LOQ)is 0.12 mg/g.The relative standard deviation(RSD)is 0.43％.Within three concentration levels(1.0,3.0,10.0 mg/g),the recovery rate ranges is from 95.0％to 98.1％.This method is fast,accurate,and stable,and can meet the determination of inositol content in vitamin premixes.
gas chromatographyvitamin premixinositolsilylation derivatization
万方数据
维普
