Objective:To establish the method to deteminate the content of polysaccharide in Abelmoschi Corolla,it may provide the basis for quality control of Abelmoschi Corolla.Methods:Phenol-sulfu-ricacid method was used for chromogenic recction.The content of total polysaccharide was measured by UV spectrophotometry at 485 nm.Results:The total polysaccharides calibration curve was at the range of 2.270～13.620 μg/mL,with regression function being Y=0.061 218 6X-0.017 805 8(r=0.999).The recowery of calycosin was 99.15%,RSD为 0.66%(n=9).Conclusion:The phenolsulfuric acid method is stable,reliable,simple and easy to operate in the determination of polysaccharides in Abelmoschi Corolla.
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