Optimization of Ultrasonic Assisted Extraction and Antioxidant Activity of Polysaccharides from Ganoderma Lucidum Spore Powder Dregs
Object:Optimization of extraction process of polysaccharides from Ganoderma lucidum spore powder dregs.Methods:Box-Behnken test design explores the effect of liquid-solid ratio,extraction time and extraction temperature on the extraction rate of polysaccharide.Determine the optimal extraction conditions to optimize the extraction efficiency of polysaccharides,and study the antioxidant activity of the residue polysaccharide in vitro.Results:In the study of polysaccharide extraction process in the residue of Ganoderma lucidum spore powder,we determined the ultrasonic power of 300 W,liquid-solid ratio of 120∶1(mL·g),extraction time of 30 min and extraction temperature of 65 ℃.Under this extraction condition,the polysaccharide extraction rate of Ganoderma lucidum spore powder residue reached 6.31％;the cell model of oxidative stress showed that it has some antioxidant capacity.Conclusions:In the extraction process of ganoderma spore powder residue,the ultrasonic extraction method can effectively and fully release the polysaccharide components.These polysaccharides significantly increased the activity of glutathione peroxidase,superoxide dismutase,and catalase.In addition,they have also actively promoted the growth of glutathione content,further confirming the diversity of their biological activities.More importantly,the extraction and application of polysaccharides performs well in reducing the content of malondialdehyde,providing new possibilities for research and application in related fields,thus demonstrating its unique biological effects and wide application prospects.
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