Study on Extraction of Coenzyme Q10 from Hair by Direct Reflux Method
Direct reflux process was used to extract coenzyme Q1o from hair,and the influence of different extraction conditions on the extraction amount of Coenzyme Q10 was studied.Orthogonal test was used to optimize the solid-liquid ratio,temperature and time of the extraction solution,so as to determine the best extraction conditions of coenzyme Q1o from hair.The results showed that the optimal extraction conditions were solid-liquid ratio 1∶10(g∶mL),temperature 80 ℃ and time 120 min with anhydrous ethanol as extraction reagent.Under these conditions,the maximum amount of coenzyme Q10 extracted from hair was 0.726 3 μg/g,and the RSD was 1.86％.The average recovery of Coenzyme Q1o was 94.21％and RSD was 4.42％.
direct refluxhaircoenzyme Q1ohigh performance liquid chromatography
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