Study on the Extraction Technology of Total Flavones from the Centralization of Guizhou Polygonum Perfoliatum L
Objective:The extraction technology of total flavonoids from Guizhou produced Polygonum perfoliatum L was studied,and the best extraction technology was found.Method:Two conventional extraction methods,ethanol thermal reflux method and ultrasonic extraction method,were used for extraction.The process with higher extraction rate was selected,and then combined with enzymatic hydrolysis to extract again.Combined with enzymatic hydrolysis method,the optimal enzymatic conditions were obtained by single factor experiment,and the optimal combination was optimized by orthogonal test.Result:The optimal extraction process isultrasound assisted enzymatic hydrolysis.The optimal extraction conditions are enzymatic hydrolysis temperature of 50 ℃,enzymatic hydrolysis pH of 6,enzymatic hydrolysis time of 100 minutes,and cellulase concentration of 0.3 mg·mL-1.Conclusion:The total flavonoids from Guizhou produced Polygonum perfoliatum L were extracted by ultrasonic-assisted enzymatic method.This process is simple,highly reproducible,and has a high extraction rate,and can be used for largescale extraction of total flavonoids in Polygonum perfoliatum L from Guizhou province.
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