GC Analysis and Methodology Research for Enantiomer in(R)-(-)-Glycidyl Butyrate
A GC method has been established for detecting enantiomer((S)-(+)-Glycidyl butyrate)in(R)-(-)-Glycidyl butyrate.The detector is a hydrogen flame ionization detector,with a temperature set at 300 ℃;The temperature of injection port is 220 ℃;A SPB-20(20％phenyl-80％dimethylpolysiloxane)containing 25％2,3-di-O-acetyl-6-O-TBDMS-β-Capillary column with cyclodextrin as stationary liquid(Superlco Beta DEX 225,30 m × 0.25 mm,0.25 μm)was used.The initial column temperature is 60 ℃,maintained for 3 minutes,and heated at a rate of 5 ℃ per minute to 200 ℃,maintained for 5 minutes;The split ratio is 50∶1;The carrier gas is nitrogen,and the flow rate is 2.5 mL per minute.The injection volume is 1 μL.The limit of quantitation of(S)-(+)-Glycidyl butyrate is 7.5 μg/mL,the method was linear from 7.5 μg/mL to 100 µg/mL,the accuracy was 96.2％～103.1％in three concentration levels from 10 µg/mL to 100 µg/mL.The method has strong specificity,high sensitivity and accuracy,good precision and durability,and can be used for the quality control of(R)-(-)-Glycidyl butyrate.
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