Detection of Fe3+in Vivo by Red Fluorescent Probes Based on Carbon Dots
In this study,red fluorescence carbon quantum dots(RCDs)were prepared using dopamine hydrochloride,citric acid and formamide.The structures of RCDs were characterized by infrared spectroscopy,indicating that the structures contain carboxyl and imino groups.The optical properties of CDs were tested by UV-visible absorption spectrophotometer and fluorescence spectrometer.The results showed that the absorption peak of RCDS was 550 nm,the emission wavelength was 593 nm,and it was a red fluorescent carbon quantum dot.The stability of RCDs shows high stability in ionic solutions and different pH solutions,and has good selectivity for Fe3+.In addition,the fluorescence intensity of RCDs is linearly related to Fe3+concentration within a certain range,indicating that CDs has strong sensitivity to Fe3+.Finally,the fluorescence of RCDs in cells was also quenched after the addition of Fe3+in cell imaging,indicating that the probe can be used for the detection of Fe3+in vivo.
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