Study on the Identification of Standard Decoction and Water Extract of Eriocauli Flos and Eriocaulon.sexangulare L
Objective:The UPLC characteristic map and thin layer identification method of Eriocauli flos standard decoction were established.lt is used to distinguish standard decoction,formula granule and counterfeit standard decoction of Eriocaulon.sexangulare L.Methods:The standard decoction of Eriocauli flos and Eriocaulon.sexangulare L was determined by UPLC characteristic map and thin layer map,10 batches of Eriocauli flos standard decoction and 5 batches of Eriocaulon.sexangulare L standard decoction were qualitatively analyzed by TLC behavior comparison,similarity analysis,cluster analysis(CA),partial least square discriminant(PLS-DA)and principal component analysis(PCA).Results:The UPLC characteristic maps with better separation and repeatability were obtained,and both the characteristic maps and thin layer identification could distinguish the two species well.Conclusion:The characteristic map is simple and accurate,with high sensitivity and good repeatability.Thin-layer identification is simple,quick and reproducible,and can be used as a quality control method for herbal medicines,decoction pieces,standard decoction and formula granules.