首页|谷精草与华南谷精草标准汤剂及其水提物的鉴别研究

谷精草与华南谷精草标准汤剂及其水提物的鉴别研究

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目的:建立谷精草标准汤剂的UPLC特征图谱和薄层鉴别方法,用于谷精草标准汤剂、配方颗粒与伪品华南谷精草标准汤剂的区分.方法:采用UPLC特征图谱及薄层图谱对谷精草、华南谷精草标准汤剂进行测定,通过薄层图谱行为比对,相似度分析、聚类分析(CA)、主成分分析(PCA)、偏最小二乘判别法(PLS-DA)对10批谷精草标准汤剂及5批华南谷精草标准汤剂进行定性分析.结果:得到分离度、重复性均较好的谷精草UPLC特征图谱,特征图谱与薄层鉴别均能较好地区分谷精草与华南谷精草.结论:特征图谱简便准确、灵敏度高、重复性好;薄层鉴别操作简单快捷,重现性好,可以作为谷精草药材、饮片、标准汤剂、配方颗粒的质量控制方法.
Study on the Identification of Standard Decoction and Water Extract of Eriocauli Flos and Eriocaulon.sexangulare L
Objective:The UPLC characteristic map and thin layer identification method of Eriocauli flos standard decoction were established.lt is used to distinguish standard decoction,formula granule and counterfeit standard decoction of Eriocaulon.sexangulare L.Methods:The standard decoction of Eriocauli flos and Eriocaulon.sexangulare L was determined by UPLC characteristic map and thin layer map,10 batches of Eriocauli flos standard decoction and 5 batches of Eriocaulon.sexangulare L standard decoction were qualitatively analyzed by TLC behavior comparison,similarity analysis,cluster analysis(CA),partial least square discriminant(PLS-DA)and principal component analysis(PCA).Results:The UPLC characteristic maps with better separation and repeatability were obtained,and both the characteristic maps and thin layer identification could distinguish the two species well.Conclusion:The characteristic map is simple and accurate,with high sensitivity and good repeatability.Thin-layer identification is simple,quick and reproducible,and can be used as a quality control method for herbal medicines,decoction pieces,standard decoction and formula granules.

Eriocauli flosspecific chromatogramthin layer chromatographystandard decoctionEriocaulon.sexangulare

李泽龙、孙海涛、程立伟、闫晓鑫、周永康、张志强

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中药配方颗粒关键技术国家地方联合工程研究中心,天津 301700

北京市中药配方颗粒工程技术研究中心,北京 101301

北京康仁堂药业有限公司,北京 101301

谷精草 特征图谱 薄层色谱 标准汤剂 华南谷精草

2024

山东化工
山东省化工研究院 山东省化工信息中心

山东化工

影响因子:0.249
ISSN:1008-021X
年,卷(期):2024.53(21)