Determination of 10 Psychoactive Substances in Hair by UPLC-MS/MS
An ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was developed for the simultaneous determination of 10 psychoactive substances in hair.A rapid and convenient wet freezing grinding method combined with ultrasonic extraction was used to extract 10 psychoactive substances in hair.The samples were separated by Kinetic Biphenyl(100 mm×2.1 mm,1.7 μm)and detected by multiple reaction monitoring(MRM)mode under positive ion mode(ESI+)of electrospray ion source.The results showed that the detection limits of 10 psychotropic substances in hair were between 0.001～0.006 ng/mg,and the quantitative limits were between 0.003～0.001 9 ng/mg.The linear relationship was good in the range of 0.02～2 ng/mg,and R2 was greater than 0.994 3.In the low,medium and high(0.02,0.2,2 ng/mg)concentrations,the matrix effect was between 84.53％and 112.01％,the extraction recovery was between 88.68％and 112.88％,and the intra-day and inter-day precision RSD were 1.48％～6.32％and 1.25％～7.85％,respectively.The method has good specificity,high sensitivity and good accuracy,and can be used for rapid and accurate determination of 10 psychoactive substances in hair.
hairpsychoactive substancespublic securityultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
万方数据
维普
