摘要
研究红果樫木叶的化学成分,运用大孔树脂、硅胶、Sephadex LH-20 和半制备色谱等多种色谱技术从红果樫木叶70% 乙醇提取物中分离得到 4 个木脂素类化合物.运用1H NMR(核磁共振)、13 C NMR和MS(质谱)波谱学数据等分别鉴定为 4,4′-dihydroxy-3,3′,5,5′-trtramethoxy-7,9′:7′,9-diepoxylignane(1),4′-hydroxy-3,4,5,3′-trtramethoxy-7,9′:7′,9-diepoxylignane(2),4′,4″-dihydroxy-3,3′,3″,5,5′,5″-hexamethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineolignan-7″,9″-diol(3)和 4′,4″-dihydroxy-3,3′,3″,5,5′-pentamethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineolignan-7″,9″-diol(4).化合物 1~4 均首次从樫木属植物中分离得到.使用DPPH(1,1-二苯基-2-三硝基苯肼)自由基清除率测定化合物的抗氧化活性,结果显示木脂素类化合物 1~4 具有良好的DPPH自由基清除能力.
Abstract
To study the constituents of the leaves of Dysoxylum gotadhora,the 70% ethanol extract was separated using macroporous resin column chromatography,silica gel column chromatography,Sephadex LH-20 gel column chromatography,combined with semi-preparative HPLC and other methods.Four compounds 4,4′-dihydroxy-3,3′,5,5′-trtramethoxy-7,9′:7′,9-diepoxylignane(1),4′-hydroxy-3,4,5,3′-trtramethoxy-7,9′:7′,9-diepoxylignane(2),4′,4″-dihydroxy-3,3′,3″,5,5′,5″-hexamethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineolignan-7″,9″-diol(3),4′,4″-dihydroxy-3,3′,3″,5,5′-pentamethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineo lignan-7″,9″-diol(4)were isolated.Their structures were established by 1H NMR,13C NMR and MS spectroscopy techniques.Compounds 1 to 4 were obtained from the genus Dysoxylum for the first time.DPPH(1,1-diphenyl-2-picryhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl)radical scavenging rate were used to determine the antioxidant activity.The results show that lignans have significant DPPH free radical scavenging ability.
基金项目
国家自然科学基金(32200311)
济南市高等学校20条项目(202228020)
齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2022PX029)
维普
万方数据