摘要
以蛋黄卵磷脂、胆固醇和神经酰胺为成膜材料,采用薄膜分散法制备胶原蛋白神经酰胺脂质体(CO-CS).以包封率为响应值,以药脂比、膜材比、超声时间和水合时间为影响因素对工艺进行优化.结果表明,当药脂比为 1:16、超声时间为 8.5 min、膜材比为 4.3:1 时,包封率可达 90.73% .在此条件下,样品的粒径为(206.63±2.06)nm,聚合物分散性指数(polymer dispersity index,PDI)为0.187±0.010,Zeta电位为(－34.90±0.82)mV.避光保存60d后,CO-CS的包封率为87.2%,粒径为(223.70±1.85)nm、PDI为 0.174±0.013、Zeta电位为(－33.51±2.10)mV.结果表明,本研究制备的CO-CS包封率高,稳定性良好,制备工艺合理可行.
Abstract
In this study,egg yolk lecithin,cholesterol,and ceramide were used as film-forming materials to prepare collagen ceramide liposomes(CO-CS)by the thin film dispersion method.To optimize the process,encapsulation efficiency was used as the response value,and it was evaluated by influencing factors such as drug-lipid ratio,film-material ratio,ultrasonic time,and hydration time.Results showed that encapsulation efficiency reached 90.73% when the drug-lipid ratio,film-material ratio,and ultrasonic time were 1:16,4.3:1,and 8.5 min,respectively.Under these conditions,the particle size,polymer dispersity index(PDI),and zeta potential of CO-CS were(206.63±2.06)nm,0.187±0.010,and(34.90±0.82)mV,respectively.After 60 days of storage at room temperature in the dark,the encapsulation efficiency was 87.2% .Furthermore,the particle size,PDI,and zeta potential of CO-CS changed to(223.70±1.85)nm,0.174±0.013,and(33.51±2.10)mV,respectively.In summary,CO-CS produced in this study revealed high encapsulation efficiency and good stability,and the preparation process is reasonable and feasible.
维普
万方数据