Exploration of antihyperlipidemia mechanism of Monopterus albus peptides based on hyperlipidemic zebrafish model and network pharmacology
This study aimed to explore the material basis and mechanism of action of Monopterus albus peptides against hyperlipidemia using hyperlipidemic zebrafish model and network pharmacology.Analysis of the ameliorating effects of Monopterus albus peptides on hyperlipidemia was conducted by constructing a hyperlipidemic zebrafish model and measuring the dye staining signal intensity of triglyceride and cholesterol fluorescence intensity changes.Monopterus albus peptides and disease targets were filtered using BIOPEP-UWM,SwissTargetPrediction,GeneCards,STRING,and other databases.Target GO functional enrichment analysis and KEGG pathway enrichment analysis were performed using the DAVID bioinformatics software,and the Monopterus albus peptides-potential target-signaling pathway network and theprotein-protein interaction(PPI)network were constructed.The results showed that Monopterus albus peptides at mass concentrations of 62.5 μg/mL and 125.0 μg/mL significantly reduced the intensity of the triglyceride staining signal(p<0.01)and cholesterol fluorescence intensity(p<0.001)in the vasculature of hyperlipidemic zebrafish.The results of network pharmacology showed that there were 35 potentially active peptide sequences in Monopterus albus peptides,and 21 core targets were obtained by protein interaction analysis.GO and KEGG enrichment analyses indicated that Monopterus albus peptides were mainly involved in biological processes such asregulation of lipid metabolic processes,and exertan antihyperlipidemia effect viainflammation regulation,insulin resistance,and lipid and atherosclerosis channels.Initially,this study confirmed that Monopterus albus peptides have an antihyperlipidemia effect,and revealed that Monopterus albus peptides have multiple active peptide fragments,multiple targets,and multiple channels in the biological process of reducing blood lipid levels,which will provide a theoretical basis and reference for further in-depth research on the material basis of Monopterus albus peptides and their role and application in countering hyperlipidemia.
Monopterus albus peptideshyperlipidemiazebrafishnetwork pharmacologymechanism of action
万方数据
维普
