A study on the quality standards of Huanglian Hupo Qingxin pills
To establish a method for the qualitative and quantitative assay of Huanglian Hupo Qingxin pills and provide abasis for the subsequent establishment of quality standards,the six ingredients in the formula(Aquilariae Lignum Resinatum,Fructus Choerospondiatis,Terminalia Chebula,CoptidisRhizoma,Flos Gossampini,and Semen Myristicae)were identified through microscopical observation.Thin-layer chromatography was used to identify Terminalia Chebula.Berberine hydrochloride concentration was assessed using high-performance liquid chromatography.Microscopical and thin-layer identifications of Huanglian Hupo Qingxin pills were conducted,which revealed a berberine hydrochloride concentration of 0.018 0～0.902 0 mg/mL,exhibiting a good linear relationship.The assay method was found to bescientific,sensitive,and reproducible,with relative standard deviations(RSDs)for precision,stability,and reproducibility tests all below 3.0%.The average recovery rate from spike-and-recovery testing was 101.29%,with an RSD of 2.30%.The established quality standardis simple and reproducible and can be used for studying the quality standards of Huanglian Hupo Qingxin pills.
国家科技期刊平台
NSTL
万方数据
