Characterization and identification of chemical constituents in Sedum sarmentosum Bung based on UHPLC-Q-Exactive Orbitrap-MS technology
To develop an ultra-performance liquid chromatograpy-quadrupole electrostatic field orbitrap high-resolution mass spectrometry(UHPLC-Q-Exactive Orbitrap-MS)method for characterization and identification of chemical constituents in Sedum sarmentosum Bung.The separation was performed using a Phenomenex Kinetex C18 column(2.1 mm×100 mm,2.6 μm)with a mobile phase comprising acetonitrile and water containing 0.1％formic acid via gradient elution at a flow rate of 0.3 mL/min;the injection volume was 3.0 μL.Mass spectrometry was performed in positive and negative ionization modes with electrospray ionization.The chemical constituents were characterized and identified based on retention time,precise molecular weight,fragment ions,and comparison with the reference substances.The results of this study showed that 53 compounds were characterized and identified in Sedum sarmentosum Bung,including 25 flavonoids,12 megastigmanes,11 organic acids,3 alkaloids and 2 other compounds.Five of these compounds were identified via comparison with reference compounds,and three compounds may have not been reported from Sedum sarmentosum Bung.The UHPLC-Q-Exactive Orbitrap-MS method proposed in this study can rapidly and comprehensively characterize the chemical constituents in Sedum sarmentosum Bung and provide a reference basis for the research on quality control and atherapeutic material basis of Sedum sarmentosum Bung.
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