Mechanism of action indole acetylated high-amylose maize starch in improving ulcerative colitis
The aim of this study is to investigate the mechanism action of indole acetylated high-amylose maize starch in improving ulcerative colitis.Dextran sulfate sodium salt was used to induce acute ulcerative colitis in mice.Indole acetylated high-amylose maize starch was then used to target the colon with the delivery of indole-3-acetic acid.This was combined with the administration of an aryl hydrocarbon receptor inhibitor to analyze the mice in terms of colon length;disease activity index;and levels of interleukin-6,interleukin-10,transforming growth factor β1,myeloperoxidase,and interleukin-22 in the colon,which were detected by enzyme-linked immunosorbent assay.Quantitative polymerase chain reaction was used to analyze the relative expression levels of the colonic tight junction proteins occludin,ZO-1,and CYP1A1.Flow cytometry was used to test the ratio of regulatory T cells and T helper 17 cells in the mesenteric lymph nodes.The results showed that administration of indole acetylated high-amylose maize starch significantly alleviated the shortening of the colon length;significantly reduced the disease activity index and the levels of interleukin-6 and myeloperoxidase;significantly promoted the expression of interleukin-10,transforming growth factor β1,interleukin-22,CYP1A1,occludin,and ZO-1;significantly increased the proportion of regulatory T cells;and significantly reduced the proportion of T helper 17 cells.Administration of the aryl hydrocarbon receptor inhibitor weakened the effect of indole acetylated high-amylose maize starch.These results together suggest that acetylated high-amylose maize starch can improve ulcerative colitis by activating aryl hydrocarbon receptors.
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