Effects of Dioscorea opposita Thunb.polysacchariades on exercise-related fatigue in mice through the PI3K/AKT/Gsk-3β pathway
This study aimed to investigate the antifatigue effects and mechanisms of Dioscorea opposita Thunb.polysaccharides(DTPs)on exercise-related fatigue in mice.In a mouse model of exercise fatigue where mice were subjected to exhaustive swimming and fatigue rotarod,changes in exhaustive swimming duration,body weight,fatigue metabolite accumulation,activity levels of related enzymes,liver tissue structure,and muscle glycogen(MuG)and liver glycogen(LG)contents were assessed.The results demonstrated that DTPs significantly prolonged the exhaustive swimming duration and reduced body weight loss,hepatic damage,and serum lactic acid(LD),blood urea nitrogen(BUN),and lactate dehydrogenase(LDH)levels in the mice.DTPs also significantly enhanced superoxide dismutase(SOD)activity and MuG and LG contents and significantly reduced aspartate aminotransferase(AST)levels.Additionally,in mice administered DTPs,the mRNA expression of phosphoinositide 3-kinase(PI3K)was significantly upregulated,whereas that of protein kinase B(AKT)and glycogen synthase kinase-3(Gsk-3β)was significantly downregulated in skeletal muscle.Taken together,DTPs can reduce metabolite accumulation and ameliorate exercise fatigue by regulating the PI3K/AKT/Gsk-3β pathway to promote glycogen synthesis.
国家科技期刊平台
NSTL
万方数据
