Poplar bark rot is one of the main poplar diseases threatening the safety of Three-North Shelterbelt Program in China.The biocontrol bacterial strain SF3 was isolated from the soil around the roots of poplar tree using a plate dilution method in our laboratory.The plate test showed that the bacterial strain SF3 and bacterial filtrate had a 76％(7d)inhibition rate against the pathogen;The strain was identified as Bacillus velezensis based on its morphological,physiological and biochemical characteristics,and 16S rDNA sequence analysis.The optimal fermentation conditions were pH 7～8,strain age 4 days,temperature 20～30℃,and fermentation time 3～4 days.The bacterial filtrate was not resistant to acid,alkali,or high temperature.Bacillus velezensis SF3 has strong antibacterial activity and has high prospects for industrial development and field application.
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