Identification of Antagonistic Bacteria Against Poplar Skin Rot and Study on the Antibacterial Effect of Sterile Filtrate
Poplar bark rot is one of the main poplar diseases threatening the safety of Three-North Shelterbelt Program in China.The biocontrol bacterial strain SF3 was isolated from the soil around the roots of poplar tree using a plate dilution method in our laboratory.The plate test showed that the bacterial strain SF3 and bacterial filtrate had a 76％(7d)inhibition rate against the pathogen;The strain was identified as Bacillus velezensis based on its morphological,physiological and biochemical characteristics,and 16S rDNA sequence analysis.The optimal fermentation conditions were pH 7～8,strain age 4 days,temperature 20～30℃,and fermentation time 3～4 days.The bacterial filtrate was not resistant to acid,alkali,or high temperature.Bacillus velezensis SF3 has strong antibacterial activity and has high prospects for industrial development and field application.
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