拟南芥损伤诱导的钙信号传递模式的研究
Wound-induced calcium signal transmission patterns in Arabidopsis thaliana
冯长鑫 1刘禹卿 1王晓 1蒋亚云 1陈洁婷 1侯聪聪 1李乐攻1
作者信息
- 1. 首都师范大学生命科学学院,北京 100048
- 折叠
摘要
为了研究植物系统性钙信号的产生和传递,本研究采用化学损伤和机械损伤等刺激方式,利用能够实时记录细胞质中游离钙离子浓度变化的GCaMP6s荧光指示器,观察并比较拟南芥叶片受到刺激后产生的钙离子信号变化,系统地记录和分析不同方式诱导的钙离子信号在植物体内的长距离传递模式.结果显示:水滴加到拟南芥叶表面会引起较为微弱的钙信号产生,沿叶片发育顺序传递;化学损伤和机械损伤刺激诱导的钙信号主要由损伤组织产生并传递至临近叶片,其中强酸刺激和切除叶片时,植物体内钙离子信号的长距离传递速度较快.不同类型的损伤信号导致植物钙信号在长距离传递的速度、强度和模式上存在差异,表明植物采用多样的长距离信号传导方式适应环境变化.
Abstract
In order to investigate the generation and transmission of systemic calcium signals in plants,this study used different damage methods,such as chemical damage and mechanical damage.The GCaMP6s fluorescent indicator,which can record the real-time changes in free calcium ion concentration in the cytoplasm,was used to observe and compare the changes in calcium ion signals produced by Arabidopsis thaliana leaves after stimulation.The long-distance transmission patterns of calcium ion signals induced by different damages in the plant were systematically recorded and analyzed.The results showed that adding water droplets to the surface of Arabidopsis thaliana leaves would cause relatively weak calcium signal production,which was transmitted along the developmental sequence of the leaves.The calcium signals induced by chemical damage and mechanical damage stimuli are mainly generated by damaged tissues and transmitted to adjacent leaves.When using strong acids for chemical damage and cutting leaves for mechanical damage,the long-distance transmission speed of calcium ion signals in plants is faster than other methods.Different damage signals lead to differences in the speed,intensity,and mode of long-distance transmission of calcium signals in plants,indicating that plants adopt diverse long-distance signal transmission methods to adapt to environmental changes.
关键词
拟南芥/化学损伤/机械损伤/钙信号/长距离信号传导Key words
Arabidopsis thaliana/chemical damage/mechanical damage/calcium signals/long-distance signal transmission引用本文复制引用
基金项目
2022年首都师范大学实验室开放基金项目(2255140)
2021年首都师范大学本科生科学研究与创业行动项目(2155039)
出版年
2024
国家科技期刊平台
NSTL
万方数据