Regional holography theory lies in the holographic thinking of the connection within and between the region and the whole as well as among different regions,providing new theoretical guidance and practical ideas for the various problems and challenges faced in the process of regional development.This paper systematically sorts out the holographic theory,combines the holographic thought with regional research,elaborates the theoretical connotation and research paradigm of regional holographic theory.In practical application,this paper outlines the practical application of regional holographic theory with examples of urban acupuncture under microscopic scale,regional division of labor under macroscopic scale,and watershed governance and development.Provide theoretical and practical paths for regional studies,such as formulating regional development plans,alleviating regional development problems,and establishing harmonious inter-regional relations in the new era.