Determination of 98 Chemical Drugs Illegally Added into Herbal Tea by UPLC-MS/MS
Objective To establish a screening method for the simultaneous determination of 98 chemical drugs illegally added into herbal tea by UPLC-MS/MS,so as to provide a rapid and accurate technical means for supervision.Methods The 50％methanol solution was used for the ultrasonic extraction of sample.An ACQUITY® HSS T3(2.1 mm×100 mm,1.8 μm)column was used as the chromatographic column.The mixture of acetonitrile(containing 0.1％formic acid)-10 mmol/L ammonium formate solution(containing 0.1％formic acid)was used as the mobile phase.ESI ion sourcein positive and negative ion modes was used for the multiple reaction monitoring(MRM)scanning,and the retention time,parent ions and fragment ions were used to determination of the illegal addition of chemicals.Results All the 98 compounds showed good linear relationships in the corresponding linear ranges(r≥0.995).The recoveries of 98 compounds ranged from 60.1％to 125.8％at the concentration levels of 1.0 ng/ml,5.0 ng/ml and 25.0 ng/ml.The limits of detection were 0.0031-0.3432 μg/g and the limits of quantitation were 0.0102-1.1440 μg/g.The analysis time was 35 minutes.Conclusion The method is simple,efficient,and can be used for the screening of 98 illegal compounds with high risk in herbal tea,which provides strong technical support for the regulation of herbal tea.
万方数据
维普
