Objective To establish an ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the determination of batatasin compounds in Dioscoreae Bhizoma.Methods The samples were ultrasonically extracted with 70％methanol extraction solvent,followed by high-speed centrifugation,then were filtered with a 0.22 μm microporous membrane.Chromatographic separation was carried out on Shim-pack Velox C18 column with gradient elution.Electrospray ionization(ESI+)and multi-reaction monitoring(MRM)were used for analysis.Results Three kinds of batatasin showed good linear relationgships in the ranges of 0.500-20.000,0.050-5.000,0.100-2.000 mg/L,respectively.The average recoveries of batatasin were 85％-101％,with RSDs of 1.20％-4.74％,respectively.Conclusion The method is sensitive and accurate.It can be used for quantitative detection and analysis of batatasin content.It provides technical support for large-scale planting,product development,quality control and evaluation of Dioscoreae Rhizoma.
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