Determination of Benzalkonium Chloride and Benzalkonium Bromide in Eye Drops by Ion Chromatography
Objective To determine the contents of benzalkonium chloride and benzalkonium bromide as bacteriostatic agents in eye drops by ion chromatography.Methods The assay was performed on SH-AC-11 column(250 mmx4 mm)by conductivity detector,the eluent was 23 mmol/L potassium hydroxide solution with the flow rate of 0.6 ml/min,the suppressor current was 56 mA,and the sample injection volume was 25 μl.Results Benzalkonium chloride presented a good linearity between peak area and concentration in the range of 1.25-62.50 μg/ml(r=0.9997)with the average recovery of 100.6％(RSD=1.7％),and benzalkonium bromide presented a good linearity in the range of 1.02-51.00 μg/ml(r=0.9998)with the average recovery of 100.4％(RSD=1.6％).Conclusion This method has high accuracy and strong specificity,and can be used for the quality control of benzalkonium chloride and benzalkonium bromide in eye drops.
eye dropsbacteriostatic agention chromatographybenzalkonium chloridebenzalkonium bromidequality control
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维普
