Objective To determine the contents of benzalkonium chloride and benzalkonium bromide as bacteriostatic agents in eye drops by ion chromatography.Methods The assay was performed on SH-AC-11 column(250 mmx4 mm)by conductivity detector,the eluent was 23 mmol/L potassium hydroxide solution with the flow rate of 0.6 ml/min,the suppressor current was 56 mA,and the sample injection volume was 25 μl.Results Benzalkonium chloride presented a good linearity between peak area and concentration in the range of 1.25-62.50 μg/ml(r=0.9997)with the average recovery of 100.6％(RSD=1.7％),and benzalkonium bromide presented a good linearity in the range of 1.02-51.00 μg/ml(r=0.9998)with the average recovery of 100.4％(RSD=1.6％).Conclusion This method has high accuracy and strong specificity,and can be used for the quality control of benzalkonium chloride and benzalkonium bromide in eye drops.
关键词
滴眼剂/抑菌剂/离子色谱/苯扎氯铵/苯扎溴铵/质量控制
Key words
eye drops/bacteriostatic agent/ion chromatography/benzalkonium chloride/benzalkonium bromide/quality control
维普
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