Objective To establish a method for the determination of proline,glycine and alanine in injectable compound solution of hyaluronic acid by amino acid autoanalyzer.Methods The amino acids in the samples were separated by cation exchange chromatographic column,and reacted with ninhydrin by post-column derivatization at 135 ℃.The detection wavelength of proline was 440 nm,and that of other amino acids was 570 nm.The amino acids were quantified by external standard method.Results Proline,glycine and alanine showed good linear relationships in the range of 0.0148-0.074,0.0207-0.103 and 0.0247-0.1235 mg/ml,respectively(r2=0.9998).The recoveries were 98.2％,98.5％and 98.3％,and RSDs were 0.55％,0.22％and 1.40％;the limit of quantifications were 13.28,0.828 and 0.988 μg,respectively.Conclusion This method is repeatable,sensitive,accurate and simple,suitable for the quantitative analysis of amino acids in injectable compound solution of hyaluronic acid.
关键词
注射用透明质酸钠复合溶液/脯氨酸/甘氨酸/丙氨酸/含量测定
Key words
injectable compound solution of hyaluronic acid/proline/glycine/alanine/content determination
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